Product Name :
Phospho-ATF2 (T71) Recombinant Rabbit Monoclonal Antibody [SC05-90]
Predicted band size :
70 kDa
Observed band size :
70 kDa
Synonyms:
Activating transcription factor 2 antibody Activating transcription factor 2 splice variant ATF2 var2 antibody ATF 2 antibody Atf-2 antibody Atf2 antibody ATF2 protein antibody ATF2_HUMAN antibody cAMP Response Element Binding Protein 2 antibody cAMP response element binding protein CRE BP1 antibody cAMP response element-binding protein CRE-BP1 antibody 展开
Function :
Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q31.1.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Thr71 of Human ATF2 aa 58-94 / 505.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IF-Tissue, IP, IHC-P
Coupling :
unconjugated
Clone number:
SC05-90
RRID:
AB_3069914
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.2′-Deoxy-2′-fluoroadenosine Formula Aliquot store at -20℃ or -80℃.4-Bromobenzoic acid-d4 Chemscene Avoid repeated freeze / thaw cycles.PMID:33645444
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 55 kDa
Positive control :
Hela, NIH/3T3, SH-SY5Y, rat brain tissue, human prostate tissue, human uterus tissue, mouse colon tissue, human cervix tissue, human skin tissue, human breast tissue, human esophagus tissue, human placenta tissue, mouse large intestine tissue, Hela cells treated with 250ng/mL anisomycin for 30 minutes.